Prof. Dr. Nagy László

Beosztásai és munkahelyei:

Jelenlegi:
Egyetemi tanár, Debreceni Egyetem, Orvos- és Egészségtudományi Centrum,
általános Orvosi Kar, Biokémiai és Molekuláris Biológiai Intézet
(2006. szeptember 1-)

A Debreceni Klinikai Genomközpont szakmai vezetője,
(2001. július 1-)

Wellcome Trust International Senior Research Fellow
(2004. november 1 – 2009. október 31.)

Kitüntetések, díjak:

Szerkesztői és bírálói tevékenység:
A FEBS Letters szerkesztője (2005-)

PhD disszertációk:
Gina Clayton (University of Cambridge), opponens (2002)
Nusser Nóra (PTE), opponens (2005)
Újhelly Olga (SE), opponens (2005)
Fábián Zsolt (PTE), opponens (2006)

MTA doktori disszertációk:
Sass Miklós (ELTE), a bizottság titkára (2005)
Góth László (DE), a bizottság tagja (2007)

Részvétel szakmai testületekben:
Biosystems International SAS, tanácsadó (2005-)
MTA Sejt- és Fejlődésbiológiai Bizottság, elnök (2005-)
MTA Sejt- és Fejlődésbiológiai Bizottság, titkár (2000-2005)
OTKA Kórtani (KóR) Zsűri, tag (2005-)
OTKA Infraindividuális (IB1) Zsűri, tag (2002-2004)
DE OEC Tudományos Bizottság, tag (2000-)
DE OEC Sejtterápiás Központ Tudományos Tanácsadó testület, tag (2004-)
Gerson Lehrman Group Councils, Austin TX, USA, tag (2006-)
Nemzeti Kutatásfejlesztési Program 1.(NKFP) programtanács, tag (2002-2004)

Tagság szakmai szervezetekben:
Magyar Biokémiai Egyesület, tag 1989 óta.
Magyar Orvosi Kamara, tag 1994 óta.
Pro Scientia Aranyérmesek Társasága, tag 1995 óta.
Endocrine Society, Active Member, 2002 óta
European Macrophage and Dendritic Cell Society, tag 2002 óta
American Society of Biochemistry and Molecular Biology, tag 2003 óta
Magyar Bioinformatikai Társaság, alapító tag, elnökségi tag, 2006 óta

Konferenciaszervező tevékenység:
  -  EMBO Conference on Nuclear Receptors Nice, France 2003 (szervezőbizottsági tag)
  -  World Congress on Basic and Clinical Immunogenomics, Budapest 2004 (szimpózium szervező)
  -  EMBO Conference on Nuclear Receptors Lake Garda, Italy 2005, 2007 (szervezőbizottsági tag)
  -  Atherosclerosis and lipid peroxidation Debrecen-Hortobágy, 2005 (szervező)
  -  FEBS-IUBMB Congress Budapest, 2005 (szimpózium szervező)
  -  UD-HHMI Modern methods of gene expression detection and data integration,
     Debrecen 2006 (kurzus igazgató)

Témavezetés:

Tudományos Diákkör (TDK)
Simándi Zoltán
Farkas Anita

Diplomamunka és Tudományos Diákkör (MD vagy MSc fokozatot szerzett hallgatók)
(zárójelben a védés éve illetve a PhD tanulmányok kezdete)

Hsun Hua Chou (Salk Institute - UCSD) ) (1998)
Buslig Júlia (Kolozsvári Egyetem-DE) (2001)
Póliska Szilárd (2002) (jelenleg PhD hallgató 2004-)
Bagoly Péter (Kolozsvári Egyetem-DE) (2004)
Pap Attila (2004) (jelenleg PhD hallgató 2005-)
Széles Lajos (2003) (jelenleg PhD hallgató 2003-)
Brázda Péter (2004) (jelenleg PhD hallgató 2005-)
Szántó Attila (2001)
Paragh György (2003)
Törőcsik Dániel (2003) (jelenleg PhD hallgató 2003-)
Kónya Gabriella (2006)
Gábor Petra (2006)

További PhD hallgatók
Oros Melinda (2002-)
Karacs Péter (2004)

PhD fokozatot szerzett:
Benkő Szilvia (2004)
Szántó Attila (2005)
Bálint L. Bálint (2006)

Posztdoktor munkatársak:
Szatmári István (2001-)
Ralph Ruehl (német) (2003-2006)
Szántó Attila (2005-)
Britt Nakken (norvég) (2005-)
Bálint L. Bálint (2006-)
Rőszer Tamás (2007-)

Kutatástámogatás és ösztöndíjak:

Aktív:

International Research Scholarship of the Howard Hughes Medical Institute (USA) “PPARg a lipid activated transcription factor at the crossroad of lipid metabolism and inflammation” # 55005621
(2006-2010) USD 500,000

International Research Scholarship of the Howard Hughes Medical Institute (USA) “Role of a lipid activated transcription factor, PPARgamma in the innate responses of macrophages during pathogen infection” #5500524
(2005-2010) USD 500,000

Wellcome Trust International Senior Research Fellowship “Role of RXR heterodimers in macrophage differentiation and function” #074021
(2005-2009) GBP 450,000


Teljesített:

“Practical Course on Advanced Methods on Gene Expression Analysis”
Howard Hughes Medical Institute (USA) 150,000 USD (2005-2006)

International Research Scholarship of the Howard Hughes Medical Institute (USA)
“Role of PPARg in normal monocyte/macrophage cell function and in disease”
(2001-2005) USD 425,000

Biotechnológia 2002 (Oktatási Minisztérium)
New molecular methods for the detection and monitoring of metabolic diseases: the role of nuclear receptors (2002-2005)
HUF 40 M

Human Frontier Science Program Organization
Crosstalk between PPAR and LXR in the control of lipid metabolism
Young Investivestigator Award
(2001-2005) USD 250,000

European Union Framework Program 5 “EU-NUC-REC-NET ” Research Training Network (2000-2004) EUR 194,000

Országos Tudományos Kutatási Alapprogramok (OTKA) T034434 A PPARg:RXR hormonreceptor heterodimer szerepe myeloid sejtek differenciálódásában és funkcióiban
(2001-2004) HUF 16,4 M

Felsőoktatási Kutatási és Fejlesztési Pályázat (FKFP) A PPAR és LXR magreceptorok szerepe a módosított LDL által kifejtett biológiai hatásokban 0208/2001
(2001-2003) 9 MFt

Royal Society (UK)
Hormonal regulation of nuclear receptor co-repressor interactions
(UK project leader: Dr John W.R.Schwabe MRC-LMB, Cambridge)
(2000-2001) GBP 10,000 (=USD 16,000 )

Boehringer Ingelheim Alapítvány Kutatási Díj (1999-2002) DM 100,000

Fogarty International Research Collaboration Award (FIRCA) (1999-2002)
USD 96,000 (Dr Peter Davies-szel, University of Texas-Houston)
1RO1-CA76088

Egészségügyi Tudományos Tanács (ETT) (Hungarian Ministry of Health) T-07 254/99
A lipid aktivált transzkripciós faktor, PPAR szerepe az atheroszklerózis kialakulásában
(1999-2000) HUF 1,600,000

Leukemia Society of America Special Fellow Award (1998-1999) USD 119,100

Postdoctoral Fellowship of the Howard Hughes Medical Institute (1997-1998, 12 hónap) USD 34,000

Postdoctoral Fellowship of the University of Texas-Houston, Medical School (1992, 6 hónap)
USD 10,000

Pro Cultura Alapítvány Támogatása, Dept. of Pathology, University of Edinburgh (1991) USD 1,500


Kutatási megbízások (K+F):

Aktív:

Biosystems International SAS, France
Comprehensive pilot and biomarker early validation studies for COPD GPCR target and biomarker discovery
(2005-2007) EUR165,000

Richter RT
0980699 Global gene expression analysis on rat liver
3,225,000 HUF (2006-2007)

Teljesített:

Pfizer Global Research, Sandwitch, UK
Histopathological analysis of COPD lung tissues
(2004-2006) EUR 38,000

Pfizer Global Research, Sandwitch, UK
Discovery and validation of biomarkers and drug targets for COPD: a clinical genomics, proteomics and genetics collaboration with the University of Debrecen.
(2003-2006) EUR 340,000

N-Gene Kft., Budapest
PPARa and g activity of selected compounds
(2004) HUF 300,000

Pfizer Global Research, Fresnes, France
Identification of disease relevant target and biomarker candidates by comprehensive interrogation of the genome and proteome in COPD
(2001-2002) USD 84,000

Biorex Rt, Budapest
Kvantitaív PCR kifejlesztése (2001-2002) 2 M HUF

Közlemények:

1991

1. Retinoic Acid Receptor Transcripts in Human Umbilical Vein Endothelial Cells
Fesus, L., Nagy, L., Basilion,J. and Davies, P.J.A.
Biochem. Biophys. Res. Comm., 179:32-38 (1991)

1994

2. Tissue Transglutaminase: an effector in physiologic cell death
Nagy, L., Thomazy,V. and Davies,P.J.A.
Cancer Bulletin 46:136-140 (1994) INVITED REVIEW

1995

3. Activation of Retinoid X Receptors Induces Apoptosis in HL-60 Cell Lines
Nagy, L., Thomazy, V.A., Shipley, G.L., Fesus, L., Lamph, W., Heyman, R.A.,
Chandraratna, R.A.S. and Davies, P.J.A.
Molecular and Cellular Biology 15:3540-3551 (1995)

1996

4. Identification and Characterization of a Versatile Retinoid Response Element (Retinoic Acid Response Element/Retinoid X Receptor Response Element) in the Mouse Tissue Transglutaminase Gene Promoter
Nagy, L., Saydak, M.M., Shipley, N., Lu, S., Basilion, J.P., Yan, Z-H., Syka, P.,
Chandraratna, RA.S., Stein, J.P., Heyman, R.A. and Davies, P.J.A.
Journal of Biological Chemistry 271 (8): 4355-4365 (1996)

5. Retinoid-regulated Expression of BCL-2 and Tissue Transglutaminase During Differentiation and Apoptosis of Human Myeloid Leukemia (HL-60) Cells Nagy, L., Thomazy, V.A., Heyman, R.A., Chandraratna, R.A.S. and Davies, P.J.A. Leukemia Research 20 (6): 499-505 (1996)

6. Retinoic acid induction of the tissue transglutaminase promoter is mediated by a novel response element
Yan, H- Z., Noonan, S., Nagy, L., Davies, P.J.A. and Stein, J.P.
Molecular and Cellular Endocrinology 120: 203-212 (1996)

Ph.D. utáni közlemények:

1997

7. Nuclear receptor repression mediated by a complex containing SMRT, mSin3A and histone deacetylase
Nagy, L., Kao, H-Y., Chakravarti, D., Lin, R.J., Hassig, C.A.,
Ayer, D.E., Schreiber, S.L. and Evans, R.M.
Cell 89 (3): 373-380 (1997)

8. Lack of induction of tissue transglutaminase but activation of the preexisting enzyme in c-myc-induced apoptosis of CHO cells
Balajthy, Z., Kedei, N., Nagy, L., Davies P.J.A and Fesus., L.
Biochem. Biophys. Res. Comm. 236:280-284 (1997)

9. Nuclear receptor co-activator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300
Chen, H., Lin, R., Schiltz, L., Chakravarti, D., Nash, A., Nagy, L., Privalsky, M.L., Nakatani, Y. and Evans, R.M.
Cell 90 (3): 569-580 (1997)

10. The promoter of the mouse tissue transglutaminase gene directs tissue-specific, retinoid regulated and apoptosis linked expression
Nagy, L., Thomazy, A.V., Saydak, M.M., Stein, J.P. and Davies, P.J.A.
Cell Death and Differentiation 4 (7): 534-547 (1997)

1998

11. TNF-a modulates expression of the tissue transglutaminase gene in liver cells
Kuncio, GS., Tsyganskaya, M., Zhu, J,, Liu, S-L., Nagy, L.,
Thomazy, VA., Davies, PJA. And Zern, MA
American Journal of Physiology 37(2): G240-252 (1998)

12. Retinoid-induced apoptosis in normal and neoplastic tissues
Nagy, L., Thomazy,V.A., Heyman, R.A and Davies, P.J.A.
Cell Death and Differentiation 5(1): 11-19 (1998) INVITED REVIEW

13. A transgenic mouse model for the study of apoptosis during limb development
Nagy, L., Thomazy, V. A, and Davies, P.J.A.
Cell Death and Differentiation 5(1): 126 (1998) INVITED REVIEW

14. Role of the histone deacetylase complex in Acute Promyelocytic Leukemia
Lin, J.R., Nagy, L., Satoshi, I., Shao, W., Miller, W., and Evans, R.M.
Nature 391:811-814 (1998)

15. Oxidized LDL regulates macrophage gene expression through ligand activation of PPARg
Nagy, L., Tontonoz, P., Alvarez, JGA., Chen, H. and Evans, RM.
Cell 93(2): 229 -240 (1998)

16. PPARg promotes monocyte/macrophage differentiation and uptake of oxidized LDL
Tontonoz, P.*, Nagy, L.*, Alvarez, JGA., Thomazy, VA. and Evans, RM.
Cell 93(2): 241 - 252 (1998)
* joint first authors

1999

17. Essential roles of retinoic acid signaling in interdigital apoptosis and control of BMP-7 expression in mouse autopods
Dupe, V., Ghyselinck, N.B., Thomazy, V., Nagy, L., Davies, P.J.A., Chambon, P. and Mark, M.
Developmental Biology 208:30-43 (1999)

18. Regulation of macrophage gene expression by PPARg: implications for cardiovascular disease
Tontonoz, P and Nagy, L.
Current Opinion in Lipidology 10(6):485-490 (1999) INVITED REVIEW

19. Molecular mechanisms of nuclear hormone receptor action in health and disease Nagy, L.
B.I.F. Futura (Boehringer Ingelheim Funds) 14:257-265 (1999) INVITED REVIEW

20. Mechanism of co-repressor binding and release from nuclear hormone receptors
Nagy, L., Kao H-Y., Love, JD, , Li, C., Banayo, E., Gooch, JT., Chatterjee, VKK, Evans, RM and Schwabe, JWR
Genes and Development 13(24): 3209-3216 (1999)

2000

21. Transcriptional repression by nuclear receptors: mechanisms and role in disease
Love, J.D., Gooch, J.T., Nagy, L., Chatterjee, V.K.K. And Schwabe, J.W.R
Biochem. Soc. Trans. 28: 390-396 (2000) INVITED REVIEW

22., A role for PPARa in oxidized phospholipid induced synthesis of MCP-1 and IL-8 by endothelial cells
Lee, H, Shi, W, Tontonoz, P, Wang, S, Subbanagounder, G., Hedrick, L., Hama, S.,
Borromeo,C., Evans, RM., Berliner, JA and Nagy, L.
Circulation Research 87: 516-521 (2000)

23., Divergent signalingpathways regulate the promoter of tissue transglutaminase
Szegezdi, E, Szondy, Z, Nagy, L., Nemes, Z., Friis, RR., Davies, PJA and Fesus, L.
Cell Death and Differentiation 7(12):1225-1233 (2000)

2001

24. PPARg dependent and independent effects on macrophage gene expression in lipid metabolism and inflammation
Chawla, A., Barak, Y., Nagy, L., Liao, D. Tontonoz, P., and Evans, RM
Nature Medicine 7(1):48-53 (2001)

25. Tissue specific effects of RXR and PPAR-gamma ligands on metabolic gene expression in diabetic rodents
Ahuja, HS, Crombie, DL, Boehm, M,Leibowitz, MD, Heyman, RA, Depre, C,
Nagy, L., Tontonoz, P and Davies, P.J.A.
Molecular Pharmacology 59 (4) 765-773 (2001)

26., A PPARg-LXR-ABCA1 pathway in macrophages is involved in cholesterol efflux and atherogenesis
Chawla, A, Boisvert, W.A., Lee, C-H., Laffitte, B., Barak, Y., Joseph, S.B.,
Nagy, L., Liao, D., Edwards, P.A., Curtiss, L.K., Evans, R.M., and Tontonoz , P.
Molecular Cell 7: 161-171 (2001)

2002

27., The structural basis for the specificity of retinoid-X-receptor selective agonists: new insights into the role of helix H12.
Love, J.D., Gooch, J.T., Benko, S., Nagy, L., Chatterjee, V.K.K., Evans,, R.M. and Schwabe, J.W.R.
Journal of Biological Chemistry 277(13):11385-11391 (2002)

28., Lipid sensors in atherosclerosis: The role of nuclear hormone receptors in disease progression
Szanto, A and Nagy, L.
B.I.F. Futura (Boehringer Ingelheim Funds) 17:129-136 (2002) INVITED REVIEW

2003

29. The retinoid X receptor and its ligands: versatile regulators of metabolic function, cell differentiation and cell death
Ahuja, A.S., Szanto, A., Nagy, L. and Davies, P.J.A.
Journal of Biological Regulators and Homeostatic Agents 17:29-45 (2003) INVITED REVIEW

30. Molecular determinants of the balance between co-repressor and co-activator recruitment to the retinoic acid receptor
Benko S., Love, J.D., Beladi, M., Schwabe, J.W.R. and Nagy, L.,
Journal of Biological Chemistry 278: 43797-43806 (2003)

2004

31. The mechanism of nuclear receptor molecular switch
Nagy, L. and Schwabe J.W.R.
Trends in Biochemical Sciences 29(6):317-324 (2004)

32. Activation of PPAR-gamma specifies a dendritic cell subtype capable of enhanced induction of iNKT cell expansion
Szatmari, I., Gogolak, P., Im, S. J., Dezso, B., Rajnavolgyi, E. and Nagy, L.
Immunity 21:95-106 (2004)

33. Transcriptional regulation of human CYP27 integrates retinoid, PPAR and LXR signaling
Szanto, A., Benko, S., Szatmari, I., Balint, L.B., Furtos, I., Rühl, R., Molnar, S., Csiba, L., Garuti, R., Calandra, S., Larsson, H., Diczfalusy, U. and Nagy, L.
Molecular and Cellular Biology 24(18):8154-8166 (2004)

34. Retinoid X Receptors: X-ploring their (patho)physiological functions
Szanto A., Nakar, V., Shen, Q., Uray, I.P., Davies, P.J.A. and Nagy, L.
Cell Death and Differentiation 11:S126-S143 (2004) INVITED REVIEW

2005

35. Retinoids potentiate PPAR-gamma action in differentiation, gene expression and lipid metabolic processes in developing myeloid cells
Szanto, A and Nagy, L.
Molecular Pharmacology 67(6):1935-1943 (2005)

36. Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells
Balint L. B., Szanto, A., Madi, A., Bauer, U-M., Gabor, P., Benko, S., Puskas, L.,
Davies, P.J.A. and Nagy, L.
Molecular and Cellular Biology 25:5648-5663 (2005)

37. Genome-wide localization of histone 4 arginine 3 methylation in a differentiation primed myeloid leukemia cell line.
Balint L. B., Gabor, P. and Nagy, L.
Immunobiology 210:141-152 (2005)

38. Flow cytometric detection of intracellular coagulation factor XIII-A: its utilization in the diagnosis and monitoring of monocytic acute myeloid leukemias.
Kappelmayer, J., Simon, A., Katona, E., Szanto, A., Nagy, L., Kiss, A., Kiss, Cs. and Muszbek, L.
Thrombosis and Heamostasis 94(2):454-459 (2005)

39. Opposite expression of Factor XIII-A gene in classical and alternative activation of macrophages
Torocsik, D., Bardos, H., Nagy, L. and Adany, R.
Cellular and Molecular Life Sciences 62:2132-2139 (2005)

40. Atherosclerosis and lipid peroxidation (Editorial)
Nagy, L. and Spiteller, G.
Molecular Nutrition and Food Research 49: 989-991 (2005) EDITORIAL

41. Roles for lipid activated transcription factors
Nagy, L. and Szanto, A.
Molecular Nutrition and Food Research 49:1072-1074 (2005) INVITED REVIEW

42. Accelerated recovery of 5-fluorouracil-damaged bone marrow after
rosiglitazone treatment
Djazayeri, K., Szilvassy, Z., Peit, B., Nemeth, J., Nagy, L., Kiss, A., Szabo, B.
and Benko, I.,
European Journal of Pharmacology 522:122-129 (2005)

2006

43. SLAM/SLAM interactions inhibit CD40 induced production of inflammatory cytokine in monocyte derived dendritic cells
Rethi, B., Gogolak, P., Szatmari, I., Veres, A., Nagy, L., Rajnavolgyi, E., Terhorst, C. and Lanyi, A.
Blood 107: 2821-2829 (2006)

44. Selective modulators of PPAR activity as new therapeutic tools in metabolic diseases
Balint, L. B. and Nagy, L.
Endocrine, Metabolic and Immune Disorders-Drug Targets 6:33-43 (2006) INVITED REVIEW

45. Twenty years of nuclear receptors (Meeting report)
Nagy, L., Schüle,R., and Gronemeyer, H.
EMBO Reports 7(6): 579-584 (2006)

46. PPARg, a lipid activated transcription factor as a regulator of dendritic cell function
Szatmari, I., Rajnavolgyi, E. and Nagy, L.
Annals of the New York Academy of Sciences 1088: 207-218 (2006)
INVITED REVIEW

47. ChIP on-beads: a robust flow-cytometry based method for the evaluation of chromatin immunoprecipitation results
Szekvolgyi, L., Balint L.,B., Imre, L., Goda, K., Szabo, M., Nagy, L.,
and Szabo, G.,
Cytometry 69A:1086-1091 (2006)

48. At the crossroad of lipid metabolism and inflammation
Szeles, L., Torocsik, D. and Nagy, L.
B.I.F. Futura (Boehringer Ingelheim Funds) 21:79-85 (2006) INVITED REVIEW

49. PPARg reguleted ABCG2 expression confers cytoprotection to human dendritic cells
Szatmari, I, Vamosi, G., Brazda, P., Balint L. B., Benko, S., Szeles, L., Jeney, V., Özvegy-Laczka, G., Szanto, A., Barta, E., Balla, J., Sarkadi, B. and Nagy, L.
Journal of Biological Chemistry 281:23812-23823 (2006)

50. PPARg controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells
Szatmari, I., Pap, A., Ruehl, R., Ma, J.X., Illarionov, P.A., Besra, G.S., Rajnavolgyi, E., Dezso, B. and Nagy, L.
Journal of Experimental Medicine 203:2351-2362 (2006)

51. Non-DNA binding, dominant-negative, human PPARg mutations cause
lipodystrophic insulin resistance
Agostini, M., Schoenmakers, E., Mitchell, C.S., Szatmari, I. Savage, D., Smith,
A.G., Rajanayagam, O., Semple, R., Luan, J., L Bath, R.K., Zalin, A.N, Labib, M., Kumar, S., Simpson, H., Blom, D., Marais, D., Schwabe, J.W.R., Baroso, I., Trembath, R., Wareham, N., Nagy, L., Gurnell, M., O’Rahilly,
S. and Chatterjee, V.K.K.
Cell Metabolism 4:303-311 (2006)

2007

52. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPARg
Gogolak, P., Rethi, B., Szatmari, I., Lanyi, A., Dezso, B., Nagy, L., Rajnavolgyi, E.
Blood (in press) (2007)

53. PPARg in immunity and inflammation: cell types and diseases
Szeles, L., Torocsik, D. and Nagy, L.
BBA- Molecular and Cell Biology of Lipids (in press) (2007) INVITED REVIEW
IF (2005): 2,751